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2021-06-17

What would the complementary strand of DNA be?

What would the complementary strand of DNA be?

Between A and T there are two hydrogen bonds, while there are three between C and G. Right: two complementary strands of DNA….DNA and RNA base pair complementarity.

Nucleic Acid Nucleobases Base complement
DNA adenine(A), thymine(T), guanine(G), cytosine(C) A=T, G≡C

What is the complementary base pairing rule for DNA?

Replication relies on complementary base pairing, that is the principle explained by Chargaff’s rules: adenine (A) always bonds with thymine (T) and cytosine (C) always bonds with guanine (G).

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What does it mean when we say that the two strands of DNA are complementary to each other?

Strands are complementary to each other, its mean that if one strand contain Adenine, Gunine and cytosine so on the other strand there will Thymine bond with Adenine, Cytosine with Gunanine and Gunanine with Cytosine. That means both of the strands have opposite amino acid attached by hydrogen bonds.

How do you write a complementary strand?

The complementary strand for DNA must follow the base pairing and polarity rules. Pairing means that A=T and G=C. Polarity means that the strands have to run in opposite directions.

What happens if a nitrogen base is paired wrong?

When there is a mistake in the copying of the genetic message that is permanent, a mutation has occurred. Two of the bases in DNA (Cytosine and Thymine) are the most vulnerable, and when this happens, they may pair with each other or themselves and the message is changed.

What does guanine always bind with?

cytosine

Why won’t hydrogen bonds form between bases that are not complementary?

Two hydrogen bonds form between adenine and thymine or adenine and uracil. Complementary pairs always involve one purine and one pyrimidine base *. Pyrimidine-pyrimidine pairings do not occur because these relatively small molecules do not get close enough to form hydrogen bonds.

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Why does adenine and thymine have 2 hydrogen bonds?

In the DNA helix, the bases: adenine, cytosine, thymine and guanine are each linked with their complementary base by hydrogen bonding. Adenine pairs with thymine with 2 hydrogen bonds. The higher the temperature at which DNA denatures the more guanine and cytosine base pairs are present.

What base in DNA is paired with adenine A?

thymine

Why does complementary base pairing occur?

The nucleotides in a base pair are complementary which means their shape allows them to bond together with hydrogen bonds. The A-T pair forms two hydrogen bonds. The C-G pair forms three. The hydrogen bonding between complementary bases holds the two strands of DNA together.

How does RNA pair with DNA?

DNA and RNA bases are also held together by chemical bonds and have specific base pairing rules. In DNA/RNA base pairing, adenine (A) pairs with uracil (U), and cytosine (C) pairs with guanine (G). The conversion of DNA to mRNA occurs when an RNA polymerase makes a complementary mRNA copy of a DNA “template” sequence.

Does the base pairing change if a strand of RNA bonds with a strand of DNA?

In DNA the hydrogen bonding between A and T helps to tie the two strands together into the double helix. The base pairing of guanine (G) and cytosine (C) is just the same in DNA and RNA.

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Why is the base pairing in DNA important?

Function. Complementary base pairing is important in DNA as it allows the base pairs to be arranged in the most energetically favourable way; it is essential in forming the helical structure of DNA. It is also important in replication as it allows semiconservative replication.

What role does base pairing play in the replication of DNA?

What role does complementary base pairing play in the replication of DNA? complementary base pairing ensures that the two daughter molecules are exact copies of the parent molecule. The leading strand is initiated by an RNA primer which muse be removed and replaced with DNA, a task performed by DNA pol I.

What would the complementary strand of DNA be?

Between A and T there are two hydrogen bonds, while there are three between C and G. Right: two complementary strands of DNA….DNA and RNA base pair complementarity.

Nucleic Acid Nucleobases Base complement
DNA adenine(A), thymine(T), guanine(G), cytosine(C) A=T, G≡C

How is DNA complementary cDNA?

In genetics, complementary DNA (cDNA) is DNA synthesized from a single-stranded RNA (e.g., messenger RNA (mRNA) or microRNA (miRNA)) template in a reaction catalyzed by the enzyme reverse transcriptase. cDNA is also produced naturally by retroviruses (such as HIV-1, HIV-2, simian immunodeficiency virus, etc.)

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What would be the base sequence for the complementary DNA formed from the strand of DNA shown below?

Answer: The base sequence is GCAAT.

What does it mean to replicate a strand of DNA?

DNA replication is the process by which a double-stranded DNA molecule is copied to produce two identical DNA molecules. Replication is an essential process because, whenever a cell divides, the two new daughter cells must contain the same genetic information, or DNA, as the parent cell.

What does DNA replication end with?

The result of DNA replication is two DNA molecules consisting of one new and one old chain of nucleotides. This is why DNA replication is described as semi-conservative, half of the chain is part of the original DNA molecule, half is brand new.

What makes exact replication of DNA possible?

What makes exact DNA replication possible? The geometry of individual base pairs allows only one base to form a hydrogen bond with its complement base.

What is the first step that must occur in DNA replication?

The first step in DNA replication is the separation of the two DNA strands that make up the helix that is to be copied. DNA Helicase untwists the helix at locations called replication origins. The replication origin forms a Y shape, and is called a replication fork.

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How is DNA copied in a cell?

Replication is the process by which a double-stranded DNA molecule is copied to produce two identical DNA molecules. DNA replication is one of the most basic processes that occurs within a cell. To accomplish this, each strand of existing DNA acts as a template for replication.

What are DNA components?

DNA is made of chemical building blocks called nucleotides. These building blocks are made of three parts: a phosphate group, a sugar group and one of four types of nitrogen bases. To form a strand of DNA, nucleotides are linked into chains, with the phosphate and sugar groups alternating.

Which enzyme is responsible for unzipping the DNA double helix?

Helicase

Why must DNA strands unzip first?

To transcribe the genetic code, two nucleotide strands forming a double helix must be unwound and the complementary base pairs must be unzipped, opening a space for RNA to get access to the base pairs. The enzymatic force competing against the hydrogen bonds tries to pull apart two nucleotide strands.

What enzyme glues the new DNA strand together?

Ligase

What enzyme synthesizes the new DNA strand?

DNA polymerase

What enzyme removes RNA primer and replaces it with DNA?

DNA polymerase I

What is the result of DNA ligase’s action?

What is the result of DNA ligase’s action? DNA is broken up at specific sites. DNA translation occurs. A daughter strand of DNA produced during chromosome replication can be composed of leading and lagging strands from different replication bubbles.

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What happen if DNA ligase is absent?

DNA Ligase I Deficiency Leads to Replication-Dependent DNA Damage and Impacts Cell Morphology without Blocking Cell Cycle Progression.

Does DNA have a negative charge?

DNA is a negatively charged polymer that is made up of nucleotide building blocks. The formation of phosphodiester bonds between adjacent nucleotides forms alternating sugar and phosphate groups, called the “sugar-phosphate backbone” of a DNA molecule. Furthermore, DNA forms a double helix.

What causes DNA to have a negative charge?

The phosphate backbone of DNA is negatively charged due to the bonds created between the phosphorous atoms and the oxygen atoms. Each phosphate group contains one negatively charged oxygen atom, therefore the entire strand of DNA is negatively charged due to repeated phosphate groups.

Is DNA a positive or negative charge?

DNA is negatively charged, therefore, when an electric current is applied to the gel, DNA will migrate towards the positively charged electrode. Shorter strands of DNA move more quickly through the gel than longer strands resulting in the fragments being arranged in order of size.

Why does DNA have a slight negative charge?

DNA is negatively charged because of the presence of phosphate groups in nucleotides. The phosphate backbone of DNA is negatively charged, which is due to the presence of bonds created between the phosphorus and oxygen atoms.