What was the result of the Hershey-Chase experiment?
Hershey and Chase concluded that DNA, not protein, was the genetic material. They determined that a protective protein coat was formed around the bacteriophage, but that the internal DNA is what conferred its ability to produce progeny inside a bacterium.
How the scientists identified DNA as the genetic material?
The researchers used different radioactive elements to label the DNA and proteins in viruses. This allowed them to identify which molecule the viruses inserted into bacteria. DNA was the molecule they identified. This confirmed that DNA is the genetic material.
What was the need for using a blender and later a centrifuge during their experiments?
The bacteria infected with virus (bacteriophages) were agitated in the blender for separating viral coats from bacteria. Centrifugation later was done which resulted in the formations of a supernatant containing viral capsids and the bacteria got sedimented at the bottom.
Why did they use two types of culture media to grow viruses in explain?
(b) They used two types of culture media in order to make protein of viruses radioactive with the help of 35S in one case, and DNA molecule in virus radioactive by using 32P in the other case. This was done to identify which one of the two had entered into the bacteria during viral infection.
What were the radioactive components of medium in which viruses were grown?
Viruses grown in presence of radioactive phosphorus contained radioactive DNA but not radioactive protein as phosphorous is present only in DNA. Viruses grown on radioactive sulphur contained radioactive protein but not radioactive as DNA does not contain sulphur.
Why did Hershey and Chase used radioactive sulfur and phosphorus in their experiment?
When that cell splits, hundreds of new viruses are released. What were Hershey and Chase trying to determine in their experiments? They used radioactive isotopes of phosphorus-32 and sulfur-35 as markers so they could trace the proteins and DNA to see what they were doing to the cell.
Is Sulphur found in DNA?
They also knew that proteins contain sulfur atoms but no phosphorus, while DNA contains a great deal of phosphorus and no sulfur. They used radioactive sulfur and phosphorus to label and, so, distinguish viral proteins from viral DNA.