What type of solution is hypertonic?
A hypertonic solution is a particular type of solution that has a greater concentration of solutes on the outside of a cell when compared with the inside of a cell. This leads to water leaving the cell and flowing into the solution around it.
What are hypnotic isotonic and hypertonic solutions?
A solution will be hypertonic to a cell if its solute concentration is higher than that inside the cell, and the solutes cannot cross the membrane. If a cell is placed in a hypotonic solution, there will be a net flow of water into the cell, and the cell will gain volume.
What happens to a bacterial cell in a isotonic solution?
In an isotonic environment, both the water and solute concentration are the same inside and outside the cell and water goes into and out of the cell at an equal rate.
What effect will a hypertonic solution have on a bacteria?
If the solution is hypertonic, water from inside the bacterial cell will leave the cell, and the bacteria will shrink. The movement of water OUT of the cell is an example of osmosis.
When a bacterial cell is kept in hypotonic solution it will?
Well normally,when you place a bacterium in a hypotonic solution,it ruptures by swelling due to the osmotic gradient created by means of relatively hypertonic solution present inside the bacterial cell,but the process is relatively slower and some are totally resistant to such action by means of their cell wall …
Can bacteria survive in a hypertonic solution?
1 Answer. Hypertonic solutions dehydrate bacteria and fungi, causing cell functions to shut down.
What is a hypertonic solution used for?
Examples of when hypertonic solutions are used include to replace electrolytes (as in hyponatremia), to treat hypotonic dehydration, and to treat certain types of shock. Solutions with a lower concentration of solutes than isotonic solutions are hypotonic.
What does hypertonic solution mean?
Hypertonic solution: A solution that contains more dissolved particles (such as salt and other electrolytes) than is found in normal cells and blood. For example, hypertonic solutions are used for soaking wounds.
What diseases are caused by acid fast bacteria?
TUBERCULOSIS, LEPROSY AND OTHER DISEASES CAUSED BY ACID-FAST BACTERIA.
What bacteria are acid fast positive?
 Bacteria displaying acid fastness include:
- Genus Mycobacterium – M. leprae, M. Tuberculosis, M. smegmatis, M. Avium complex, M. kansasii.
- Genus Nocardia – N. brasiliensis, N. cyriacigeorgica, N. farcinica, and N. nova.
Why some bacteria are acid fast?
The acid-fastness of Mycobacteria is due to the high mycolic acid content of their cell walls, which is responsible for the staining pattern of poor absorption followed by high retention. Some bacteria may also be partially acid-fast, such as Nocardia.
Is Mycobacterium acid fast positive or negative?
As opposed to gram-negative or gram-positive bacteria, mycobacteria are classified as acid fast, as they exhibit low absorbance, but high retention of laboratory stains .
What stains are used in acid fast?
Acid-fast stains include the Ziehl-Neelsen stain (Fig. 11.9), the Kinyoun stain, and the Fite stain. Most practitioners choose either the Ziehl-Neelsen or the Kinyoun stain, neither of which will stain Nocardia (unlike the Fite stain).
What color is acid fast positive?
Acid Fast positive cells are stained the pink/red color of carbolfuchsin. Acid Fast negative cells are stained the light blue color of methylene blue.
Is Staphylococcus acid fast?
The small pink bacilli above are Mycobacterium smegmatis, an acid fast bacteria because they retain the primary dye. The darker staining cocci are Staphylococcus epidermidis , a non-acid fast bacterium. This is a combination of a negative stain and a simple stain.
Why is methylene blue used in acid fast staining?
So this method is also called Ziehl-Neelsen staining techniques. Neelsen in 1883 used Ziehl’s carbol-fuchsin and heat then decolorized with an acid alcohol, and counter stained with methylene blue. The main aim of this staining is to differentiate bacteria into acid fast group and non-acid fast groups.
Is E coli acid fast negative?
Purple because it is gram postive. It does have a thick peptidoglycan cell wall and therefore retains the crystal violet stain. What color is Escherichia coli under an acid fast stain? It is blue because it is acid fast negative.
Is Staphylococcus aureus acid fast positive or negative?
Staphylococci are facultative anaerobes that grow by aerobic respiration or by fermentation that yields principally lactic acid. The bacteria are catalase-positive and oxidase-negative.
Can a gram-negative bacteria be acid fast?
It actually IS possible to stain “acid-fast” bacteria with the Gram stain. The catch is that it takes 12-24 hours of staining (according to Gram’s original paper) rather than a minute or so.
Is an acid-fast bacteria a Gram positive or Gram negative bacteria?
Acid-fast bacteria are gram-positive in terms of structure because they contain peptidoglycan in the cell wall. However, they stain poorly with Gram stain procedure, appearing weakly Gram-positive.
What color would a gram negative bacteria be in an acid-fast stain?
Stains cells purple or blue. mordant, makes the dye less soluble so it adheres to cell walls. Cells remain purple or blue. Gram-positive cells remain purple or blue, Gram-negative cells are colorless.