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2021-05-14

How could you avoid bubbles in the preparation of wet mounts?

How could you avoid bubbles in the preparation of wet mounts?

Place a sample on the slide. Using a pipette, place a drop of water on the specimen. Then place on edge of the cover slip over the sample and carefully lower the cover slip into place using a toothpick or equivalent. This method will help prevent air bubbles from being trapped under the cover slip.

How do you get water bubbles out of a wet mount?

Apply a vacuum: This speeds up the movement of the fixing solution or water into the specimen. Dehydrate the specimen: Place the specimen into alcohol. Some specimens will shrink and lose water and air. By placing the specimen into water again, the specimen will take up the water.

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What is the purpose of a wet mount?

A wet mount is made by placing a fluid solution on a slide, suspending a specimen in a solution, and then covering the specimen and the solution with a cover slide. Why would use a wet mount? To increase the specimens translucency and to make it easier to stain.

What is the benefit of preparing a good wet mount?

Compared to permanently mounted slides, wet mounts do have certain advantages: Quick preparation: specimen fixation, dehydration and staining are not necessary (but possible, if required). For this reason, wet mounts are the first kind of mounts that students learn to make.

What is the advantage of the hanging drop method over the wet mount?

Advantages: Like the wet mount, the hanging drop method preserves cell shape and arrangement. The Vaseline-sealed depression also slows down the drying-out process, so the organisms can be observed for longer periods. Disadvantages: The hanging drop method is also far too risky to use with highly pathogenic organisms.

What is the value of a wet mount preparation?

What is the value of a wet-mount preparation in the clinical laboratory? The value of a wet-mount prep in the clinical lab is being able to see a living specimen and look for motility, which aids in identity of the organism. which microscope objective is most helpful when studying bacteria?

Which objective lens is the longest?

oil immersion objective lens

What are the 3 objective lenses on a microscope?

Most compound microscopes come with interchangeable lenses known as objective lenses. Objective lenses come in various magnification powers, with the most common being 4x, 10x, 40x, and 100x, also known as scanning, low power, high power, and (typically) oil immersion objectives, respectively….

What is the working distance for a 10x objective lens?

Table 2 – Long Working Distance Objectives

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Designation Magnification Working Distance
ELWD 50x 8.7 mm
ELWD 100x 2.0 mm
SLWD 10x 20.3 mm
SLWD 20x 20.5 mm

Which level of magnification has the shortest working distance?

Lab 1 Microscopy

Question Answer
This objective lens provides the highest magnification Oil immersion
This objective lens provides the second highest magnification High-dry
This objective lens provides the lowest magnification Low Power
This objectivie lens has the shortest working distance oil immersion

What happens if you try to use the coarse adjustment when the 10x lens is in place?

What happens if you try to use the coarse adjustment when the 10X lens is in place? The focus of the specimen would be unclear with a high objective power and the stage lifted high due to the coarse adjustment. “You only use the coarse focus knob when the 4X scanning objective is in place.”

Which objective has the largest working distance?

Table 2 – Long Working Distance Objectives

Designation Magnification Working Distance
SLWD 10x 20.3 mm
SLWD 20x 20.5 mm
SLWD 50x 13.8 mm
SLWD 100x 4.7 mm

Which magnification provides the largest FOV?

4x

Which objective has the smallest working distance?

Lab Test 1 part 1

Question Answer
This objective lens provides the second highest magnification? Answer: high dry
This objective lens provides the lowest magnification? Answer: low power
This objective lens has the shortest working distance? Answer: oil immersion

Which level of magnification requires the most illumination?

45x

Under which power high or low is the largest field of view seen?

The field of view is largest on the lowest power objective. When you switch to a higher power, the field of view closes in towards the center. You will see more of an object on low power.

How much more area can you see with the 4X objective?

Q: How much more area can you see with the 4x objective that with the 40x objective? i. A: When using the 4x objective one can see 2.32 more millimeters in diameter of the field of view.

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Can you see sperm at 100x?

Sperm would be hard to see at 40x. At 100x it should be visible. most likely you will not be able to focus on a sample at even moderate magnification (~40-60x) if it is between two glass slides- this is because you’ll need to bring the objective closer to the sample than the thickness of the slide will permit.

Is it more desirable to increase or decrease the light when changing to a higher magnification?

In general, the more light delivered to the objective lens, the greater the resolution. The size of the objective lens aperture (opening) decreases with increasing magnification, allowing less light to enter the objective. You will likely need to increase the light intensity at the higher magnifications.

What can you see at 400X magnification?

At 400x magnification you will be able to see bacteria, blood cells and protozoans swimming around.

At what magnification can you see sperm?

400x

What can you see with 1000x magnification?

At 1000x magnification you will be able to see 0.180mm, or 180 microns.

How much magnification do you need to see a cell?

About magnification: Most educational-quality microscopes have a 10x (10-power magnification) eyepiece and three objectives of 4x, 10x and 40x to provide magnification levels of 40x, 100x and 400x. Magnification of 400x is the minimum needed for studying cells and cell structure.

Can microscopes see DNA?

Given that DNA molecules are found inside the cells, they are too small to be seen with the naked eye. While it is possible to see the nucleus (containing DNA) using a light microscope, DNA strands/threads can only be viewed using microscopes that allow for higher resolution.