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2021-05-16

Why does chlorophyll absorb blue light the most?

Why does chlorophyll absorb blue light the most?

Violet and blue have shortest wavelength but of high energy whereas red light has longest wavelength and carry less amount of energy. The blue green color is from chlorophyll a and b reflecting those wavelengths and absorbing the rest of visible light.

What colors does chlorophyll absorb in the visible light spectrum?

Chlorophyll, the green pigment common to all photosynthetic cells, absorbs all wavelengths of visible light except green, which it reflects. This is why plants appear green to us. Black pigments absorb all wavelengths of visible light that strike them.

Is chlorophyll a water soluble?

1 Chlorophylls. Chlorophylls are green, lipid-soluble pigments, found in all algae, higher plants and cyanobacteria, which carry out photosynthesis [40].

Which of the following is a water soluble pigment?

Anthocyanins

Is chlorophyll a polar or nonpolar?

Remember, chlorophylls and carotenoids are hydrophobic or nonpolar and will dissolve in less polar solvents, whereas anthocyanins are extractable and soluble in more polar solvents like water.

Is Pheophytin more polar than chlorophyll?

Chlorophyll b and pheophytin b have one more oxygen atom around the ring than their a equivalents, making them more polar than their a equivalents, so they have smaller Rf values than their a equivalents.

Which color was attracted to the mobile phase the most?

Carotene

What did the different colored bands signify?

The different colored bands signified the different photosynthetic pigments that were inside the spinach leaf. There was a yellow-orange line present that shows a carotenoid or xanthophyll was in the leaf. The green colors present show that chlorophyll was in the leaf.

Which color has the greatest affinity for water?

blue

Why must the solvent level be below the Coloured spots in chromatography?

The solvent level has to be below the starting line of the TLC, otherwise the spots will dissolve away. Non-polar solvents will force non-polar compounds to the top of the plate, because the compounds dissolve well and do not interact with the polar stationary phase….

Why is it important the ink dots are not submerged in the solvent?

It is important that the solvent level is below the line with the spots on it. As the solvent slowly travels up the paper, the different components of the ink mixtures travel at different rates and the mixtures are separated into different colored spots….

What would be the consequences of immersing the initial spots in your filter paper with your developing solvent?

If the spots were immersed they would dissolve in the solvent and be pulled down into the solvent itself. The solvent flows through the paper, over and past the mixture of substances. As it flows, the solutes dissolve within the moving solvent and are carried along the moving solvent.

Why are the spots the same for both the right and left lanes TLC?

Questions from the experiment:Why are the spots the same for both the right and left lanes? This is because the reaction has only just begun and a complete reaction has not been yet completed.

What is the aim of co-spot in TLC?

Use of a Co-Spot TLC can be used to analyze a chemical reaction, for example to determine if the reactants have been consumed and a new product has formed. A pure sample of the reactant can be spotted in one lane of a TLC, and the product mixture in another lane….

What is a Cospot?

Co-spot means to spot compound A on one area of the TLC plate and spot the unknown on the same area as the spot of compound A. If there is only one dark spot under the UV light for the co-spotting lane, the identity of unknown is A. TLC can be used to check. Compound A and B are spotted on a TLC plate separately.

How can TLC be used to determine if a reaction has gone to completion?

At various times during the experiment, a TLC plate is spotted three times, then developed and viewed under UV light: The reaction has gone to completion, when the TLC plate shows that there is no more starting material left in the reaction mixture.

When spotting your compound on the TLC plate you don’t want to add too much of your sample what would happen to your plate if you add too much?

Don’t use too much sample When you spot a TLC plate you only want to use a little bit of a sample- one touch from a capillary tube is usually plenty. Otherwise your spots will be too big. Sometime your reaction mixture will be too concentrated to do TLC….

What happens if the solvent in TLC is too polar?

If a development solvent of too high a polarity is used, all components in the mixture will move along with the solvent and no separation will be observed (Rf’s will be too large). If the solvent is of too low a polarity the components will not move enough, and again separation will not occur (Rf’s will be too small).