What is used to create fragments of DNA?
Gel electrophoresis is a technique used to separate DNA fragments (or other macromolecules, such as RNA and proteins) based on their size and charge. Electrophoresis involves running a current through a gel containing the molecules of interest.
Which enzyme is used to bind DNA fragments together?
What are DNA restriction fragments?
A restriction fragment is a DNA fragment resulting from the cutting of a DNA strand by a restriction enzyme (restriction endonucleases), a process called restriction. A particular DNA molecule will always yield the same set of restriction fragments when exposed to the same restriction enzyme.
What is the preferred name of the technique used to determine if DNA?
What does the process of gene therapy involve? It adds a functioning version of the defective gene to the cells of an individual. What is the preferred name of the technique used to determine if DNA comes from a particular individual? DNA profiling is a forensic procedure being utilized to identify individuals.
What is an important difference between mRNA and DNA?
DNA is made up of deoxyribose sugar while mRNA is made up of ribose sugar. DNA has thymine as one of the two pyrimidines while mRNA has uracil as its pyrimidines base. DNA is present in the nucleus while mRNA diffuses into the cytoplasm after synthesis. DNA is double-stranded while mRNA is single-stranded
What is the current standard tool used for DNA profiling?
STR analysis. The system of DNA profiling used today is based on polymerase chain reaction (PCR) and uses simple sequences or short tandem repeats (STR).
What are the 4 steps of DNA profiling?
The DNA testing process is comprised of four main steps, including extraction, quantitation, amplification, and capillary electrophoresis.
What is the first step in DNA fingerprinting?
The first step of DNA fingerprinting was to extract DNA from a sample of human material, usually blood. Molecular ‘scissors’, called restriction enzymes?, were used to cut the DNA. This resulted in thousands of pieces of DNA with a variety of different lengths
Why is half of DNA replication in a discontinuous fashion?
Explain why half of DNA is replicated in a discontinuous fashion. Replication of the leading strand occurs in the direction away from the replication fork in short stretches of DNA, since access to the DNA is always from the 5′ end. This results in pieces of DNA being replicated in a discontinuous fashion.
What does semi-discontinuous mean for DNA replication?
When the double helix of DNA unwinds, DNA replication on one of the two strands (3′ to 5′ stand) can easily proceed continuously in 5′ to 3′ direction. This behaviour where the leading strand is synthesized continuously and the lagging strand is synthesized discontinuously is called semi-discontinuous replication.
On which strand of DNA replication is continuous?
On the leading strand, DNA synthesis occurs continuously. On the lagging strand, DNA synthesis restarts many times as the helix unwinds, resulting in many short fragments called “Okazaki fragments.” DNA ligase joins the Okazaki fragments together into a single DNA molecule.